Evidence for a spin-coupled binuclear iron unit at the active site of the purple acid phosphatase from beef spleen.
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Evidence for a spin-coupled binuclear iron unit at the active site of the purple acid phosphatase from beef spleen.
The purple acid phosphatase from beef spleen, which contains two iron atoms per molecule, is EPR silent in its native (oxidized) purple form. Treatment with mild reducing agents results in conversion to a pink, enzymatically active form, which exhibits an unusual EPR signal centered at g approximately equal to 1.77; double integration of the EPR spectrum gives one spin per two iron atoms. A sim...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1982
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.79.15.4623